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A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) Rev. Bras. Zool.
Ludwig,Sandra; Patella,Raquel; Stoiev,Sérgio; Castilho-Westphal,Gisela; Girotto,Marcus V. F; Ostrensky,Antonio.
Among oysters, species of Crassostrea (Sacco, 1897) are the most attractive to aquaculture. In Brazil, the genus is represented by C. rhizophorae (Guilding, 1828) and C. brasiliana (Lamarck, 1819). Because the maturation and breeding technology is not well developed for these species, aquaculturists need a reliable method to decide the correct time to place spat collectors in the field, and to identify both species, which are morphologically similar. In this study a specific Multiplex PCR protocol was developed, using one pair of universal primers from 18S rDNA as a positive control and a pair of specific primers for each target species. The sensitivity and specificity of the protocol was evaluated. It detected C. rhizophorae DNA in low concentrations, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiplex PCR; Oysters; Plankton; Specific primers.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000400002
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Duration of the pre-settlement period of the mangrove crab Ucides cordatus (Decapoda: Ocypodidae) under laboratory conditions BABT
Ventura,Robson; Silva,Ubiratã de Assis Teixeira da; Perbiche-Neves,Gilmar; Ostrensky,Antonio; Boeger,Walter Antonio Pereira; Pie,Marcio Roberto.
The goal of the present study was to determine the most appropriate time to release the immatures of Ucides cordatus (Linnaeus) produced in the laboratory into the natural environments. Specifically, the time when the megalopae sought the mangrove sediment to excavate the burrows was determined, as well as the time necessary for their metamorphosis into the first juvenile stage. Results indicated that the megalopae of U. cordatus reared in the laboratory took three to ten days (median = 6) after their molt to excavated burrows in the sediment. The average time for the megalopae to molt into juveniles was 12.6 days (SD = 2.3).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ucides cordatus; Restocking; Larval rearing; Settlement; Megalopae; Juvenile.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500012
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Fish predation on brachyuran larvae and juveniles in the Pinheiros river, Guaratuba Bay, Paraná, Brazil Rev. Bras. Zool.
Costa,Paulo V.; Silva,Ubiratã A. T. da; Ventura,Robson; Ostrensky,Antonio; Angelo,Leandro.
Fish predation is thought to exert an important influence on the demographical dynamics of larvae and juveniles of estuarine brachyuran crabs but few studies have investigated this phenomenon in nature. In this study, fishes were captured during full moons (in January and February 2005), when many brachyuran species are known to release their larvae. Samples were carried out in the Pinheiros river, Guaratuba Bay, Paraná, Brazil. The stomach contents of collected fishes were surveyed to determine the species that are most likely to prey on brachyuran immature forms in this location. Two techniques were used to capture fishes: manual samplings, using a 5-mm mesh size net (1.8m depth), and bottom trawling with a 20-mm mesh size net. The collected specimens...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crab; Megalopae; Osteichthyes; Predator; Ucides cordatus; Zoeae.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702009000200005
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Influência do cultivo de camarões marinhos em tanque-rede sobre a qualidade da água e a estrutura da comunidade zooplanctônica na Baía de Guaratuba, Paraná R. Bras. Zootec.
Angelo,Leandro; Ventura,Robson; Ostrensky,Antonio; Perbiche-Neves,Gilmar; Boeger,Walter Antonio.
Objetivou-se com este estudo avaliar os impactos ambientais causados por um sistema de cultivo de camarões marinhos em tanque-rede sobre a qualidade da água e sobre a estrutura da comunidade zooplanctônica. O estudo foi realizado na Baía de Guaratuba, onde os cultivos eram realizados em 300 tanques-rede (3,6 m de comprimento, 3,6 m de largura e 2 m de profundidade) distribuídos em uma área de 4.200 m², em densidade de 720 camarões/m². As concentrações de amônia, nitrito e fosfato na água foram monitoradas e amostras de zooplâncton foram coletadas no local dos cultivos (ponto 1) e em outros três pontos localizados a aproximadamente 200 m para oeste, noroeste e sudeste dos tanques. O ponto 5, usado como controle, localizava-se a aproximadamente 1.000 m a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Guaratuba; Impacto ambiental; Isca-viva; Litopenaeus vannamei; Zooplâncton.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001100001
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Intraspecific interactions in the mangrove crab Ucides cordatus (Decapoda: Ocypodidae) during the metamorphosis and post-metamorphosis periods under laboratory conditions Rev. Bras. Zool.
Ventura,Robson; Silva,Ubiratã A. T. da; Ostrensky,Antonio; Cottens,Kelly.
Current efforts for restocking natural populations of the mangrove crab Ucides cordatus (Linnaeus, 1763) in Brazil have focused on developing a methodology for stimulating the metamorphosis of U. cordatus larvae at a large scale. The aim of the present study was to compare the mortality rates both in individual and mass conditions, during the induction of metamorphosis of megalopa to juvenile in U. cordatus, without the use of mangrove sediment as substrate. Furthermore, the importance of intraspecific antagonistic behavior on survivorship rates during early post-metamorphosis period was investigated. Metamorphosis was induced by the use of water conditioned with conspecific adults (30 indiv. 100 L-1 for 24 hours). In the first assay, megalopae were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cannibalism; Juvenile; Megalopa; Nursery.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000100001
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Morphometric comparison between hatchery-reared and wild-caught megalopae of the mangrove crab PAB
Ayres,Bárbara Andressa Casagrande; Silva,Ubiratã Assis Teixeira da; Souza,Robson Ventura de; Boeger,Walter Antonio; Ostrensky,Antonio.
The objective of this work was to compare the morphometry of hatchery-reared and wild-caught mangrove crab (Ucides cordatus) megalopae. Ten U. cordatus megalopae of each group (hatchery-reared and wild-caught) were individually analyzed using a stereoscopic microscope equipped with an ocular micrometer. Length, width, and height of all megalopae were measured, and the size of body appendices was determined. The results indicate that the hatchery-reared megalopae are more robust than the wild ones. Furthermore, some significant differences in the size of certain appendices can be cues of the kind of alterations that hatchery-reared individuals experience.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Ucides cordatus; Body appendices; Larviculture; Rearing-environment; Restocking programs.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800056
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Ontogenetic development of tetra Astyanax lacustris (Characiformes: Characidae) Neotropical Ichthyology
Stevanato,Diego J.; Ostrensky,Antonio.
ABSTRACT Ontogenetic development of the tetra Astyanax lacustris was studied under laboratory conditions. Larvae obtained by induced reproduction were maintained individually in tissue-culture plates, at 25°C. Daily observations and morphometric measurements were performed. Larvae hatched with a total length (TL) of 3.02 ± 0.34 mm (average ± standard deviation) without pigmentation and 0.06 ± 0.02 mm³ of yolk reserves. The yolk-sac larval period lasted 26 h post-hatching (hph). During this period, eye pigmentation started, the digestive tract emerged, the anus opened, and the animals began to show steady movements. Inflation of the swimming bladder initiates the preflexion stage, which extended until 230 hph. During this stage, the transition from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax lacustris; Larval morphometry; Notochord; Ontogeny; Swim bladder.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000200206
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Prospecção do molusco invasor Limnoperna fortunei (Dunker, 1857) nos principais corpos hídricos do estado do Paraná, Brasil Pap. Avulsos de Zool. (São Paulo)
Pestana,Debora; Ostrensky,Antonio; Tschá,Marcel Kruchelski; Boeger,Walter A..
Foram realizadas coletas nos principais corpos hídricos do Paraná objetivando a prospecção de larvas do mexilhão dourado, Limnoperna fortunei (Dunker, 1857). Essa espécie de molusco vem colonizando diversos corpos hídricos na América do Sul desde sua introdução na Argentina, em 1991. Já atingiu os rios Paraná e Paraguai, tendo sua presença reportada nos estados do Mato Grosso do Sul, Paraná, São Paulo, Minas Gerais e Rio Grande do Sul. As coletas foram feitas no inverno e na primavera de 2007 e no verão e no outono de 2008, em 14 pontos amostrais ao longo dos rios Iguaçu, Piquiri, Ivaí (afluentes do rio Paraná) e Tibagi (afluente do rio Paranapanema). O presente estudo determinou a presença da espécie ao longo de todos os pontos do rio Iguaçu, em dois...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioinvasões; Mexilhão dourado; Marcadores moleculares; Rios do Paraná.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492010003400001
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Seasonal variation in larval density of Limnoperna fortunei (Bivalvia, Mytilidae) in the Iguaçu and Paraná rivers, in the region of Foz do Iguaçu, Paraná, Southern Brazil BABT
Pestana,Débora; Pie,Marcio Roberto; Ostrensky,Antonio; Boeger,Walter Antonio; Andreoli,Cleverson; Franceschi,Francisco; Lagos,Patrícia.
The larval density of the invasive mussel Limnoperna fortunei (Bivalvia, Mytilidae) was monitored from January 2005 to February 2006 at two points along the Paraná and the Iguaçu Rivers, near Foz do Iguaçu, PR, Brazil. The results indicated two density peaks in the Paraná River: a lower peak between March and June and a higher peak in October. In the Iguaçu River, the values were much lower, yet they also showed a peak between September and October. A reduction in the reproductive activity was observed in both the rivers between July and August. The average larval density at the collection point in the Paraná reached 948.5 larvae/m³, with a peak of 2,999.5 larvae/m³ in October. The present study represented the first quantification of the larval density of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Invasive species; Golden mussel; Biofouling; Larval density.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300023
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Survival of Ucides cordatus (Decapoda: Ocypodidae) megalopae during transport under different conditions of density and duration Rev. Bras. Zool.
Ventura,Robson; Silva,Ubiratã A. T. da; Ostrensky,Antonio; Cottens,Kelly; Perbiche-Neves,Gilmar.
Target areas for Ucides cordatus (Linnaeus, 1763) restocking programs are often located far from the laboratory where larval rearing is developed. During translocation, the larvae are submitted to highly stressful conditions due to handling, packing, and transport activities. The aim of the present study was to assess the mortality rates of U. cordatus megalopae caused by different transportation procedures. Megalopae at loading densities of 50, 150, and 300 ind.L-1 were packed in double polyethylene 12 x 25 cm plastic bags with 200 ml of marine water at salinity 30. The bags were filled with oxygen at a proportion of 1:2 parts of water and sealed tightly. The trepidations during transport were simulated by the use of a shaker device (800...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cannibalism; Larvae; Mangrove crab; Stress; Transport.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702010000600001
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The effect of temperature and body size on filtration rates of Limnoperna fortunei (Bivalvia, Mytilidae) under laboratory conditions BABT
Pestana,Débora; Ostrensky,Antonio; Boeger,Walter Antonio Pereira; Pie,Marcio Roberto.
The golden mussel (Limnoperna fortunei, Mollusca: Bivalvia) is an invasive species that has been causing considerable environmental and economic problems in South America. In the present study, filtration rates of L. fortunei were determined in the laboratory under different temperatures (10, 15, 20, 25, 28, and 30 ºC) and two types of food (Algamac-2000® and the chlorophycean alga Scenedesmus sp.). There was a statistically significant relationship between time and filtration rates in the experiment using Scenedesmus sp., regardless of temperature. However, this pattern was absent in the experiment using Algamac, suggesting that the relationship between filtration rates and temperature might depend on the size of the filtered particles. In addition, there...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Invasive species; Condition index; Scenedesmus sp.; Algamac-2000®; Environmental impact.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100018
Registros recuperados: 11
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